Other sea star Igkappa gene cloning assay in E. coli with new parameters
نویسندگان
چکیده
The plasmid vector pET-28b(+) named “Young” was produced according a work of 2014.1 This construct is designed to allow the expression 13.6 kDA protein with C-terminal 6histag. It supposed be an anti-HRP (Horse-radish peroxydase protein). not expressed in first E. coli: we attempt explain this phenomenon.
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ژورنال
عنوان ژورنال: Journal of Stem Cell Research & Therapeutics
سال: 2023
ISSN: ['2475-5540']
DOI: https://doi.org/10.15406/jsrt.2023.08.00159